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Polymerase Chain Reaction, PCR

Polymerase Chain Reaction

Polymerase Chain Reaction, PCR, is a scientific technique used in molecular biology to amplify specific regions of a DNA strand (the DNA target).

Online available information resources on polymerase chain reactions, PCR.

Further information categories about related topics are listed in the navigation menu on the left side of these page.


Current Stories and Reports

Polymerase Chain Reaction
Universal quenching probe system: faster, more cost-effective DNA test for crime scenes, disease diagnosis - [e]


General Information

PCR Additives
List of the more popular of additives - [e]

PCR and multiplex PCR guide
PCR optimization - [e]

PCR Primer Design and Reaction Optimisation
Molecular Biology Techniques Manual - Format: PDF - [e]

PCR Protocols
Quantitative Reverse Transcription Polymerase Chain Reaction (RT-PCR) and Other PCR Procedures - [e]

PCR techniques
An overview - [e]

PCR Troubleshooting Tips
PDF sheet - [e]

PCR Troubleshooting Tips
Troubleshooting for PCR and multiplex PCR - [e]

PCR: an outstanding method
PDF booklet - [e]

Polymerase Chain Reaction
Animated Tutorial - [e]

Polymerase Chain Reaction
Xeroxing DNA. Article, graphics gallery, and about Kary B. Mullis - [e]

Polymerase Chain Reaction
... Cloning DNA in the Test Tube. Article - [e]

Polymerase Chain Reaction (PCR)
Replicating Millions of Copies From a Single Gene. Biology 100 Laboratory Manual Exercise - [e]

Primer Design
Primers are designed to have a sequence which is the reverse complement of a region of template or target DNA to which we wish the primer to anneal. Article - [e]

Principle of the PCR
Introduction - [e]


Special Information

A critical review of PCR primer design algorithms and crosshybridization case study
PDF article by F. John Burpo - [e]

Bioinformatic tools and guideline for PCR primer design
PDF article: African Journal of Biotechnology Vol. 2 (5), pp. 91-95, May 2003 - [e]

Case Study:
The effects of business practices, licensing, and intellectual property on development and dissemination of the polymerase chain reaction. PDF article - [e]

PCR fidelity of Pfu DNA polymerase
... and other thermostable DNA polymerases. PDF Article, Nucleic Acids Research, 1996 - [e]

The enhancement of PCR amplification by low molecular
PDF article - [e]

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Polymerase Chain Reaction, PCR
Polymerase Chain Reaction, PCR, information, data, mechanism, techniques, research
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25.02.2012 [site update]

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Related Books and Scientific Literature: Polymerase Chain Reaction:


Elizabeth van Pelt-Verkuil, Alex van Belkum, John P. Hays

Principles and Technical Aspects of PCR Amplification

Kary Mullis was awarded a Nobel Prize for inventing the PCR technique more than 15 years ago in 1993. Since its "discovery", multiple adaptations and variations of the standard PCR technique have been described, with many of these adaptations and variations currently being used in clinical, diagnostic and academic laboratories across the world. Further, these techniques are being applied at the diagnostic level (e.g. as high throughput testing methodologies to detect minimum residual disease, the presence/absence of specific pathogens etc), as well as to increase our understanding of fundamental disease processes.

Frequently, PCR technicians and specialists limit their understanding of PCR to one particular methodology. However, this approach limits their appreciation of the range of versatile PCR techniques currently available, techniques that may be applicable and indeed more suitable to their own laboratory situation.

This manual aims to provide the reader with a guide to the standard PCR technique and its many available modifications, with particular emphasis on the role of PCR techniques in the diagnostic laboratory (the central theme of this manual). Further, many important technical issues have been addressed, including types of PCR template material, PCR optimization, the analysis of PCR products, quality control and quality assurance, variants and adaptations of the standard PCR protocol, quantitative PCR and in situ PCR. The reader of this manual will be excellently informed about the fundamental principles of PCR and the true potential of PCR within clinical laboratory practice.

Springer; 2008

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