High Performance Liquid Chromatography.

HPLC - high-performance liquid chromatography, or high pressure liquid chromatography - is a chromatographic technique to separate, identify, and quantify chemical compounds.

Online available information resources on High Performance Liquid Chromatography, HPLC.


General Information
Lecture Notes, Tutorials
Special Information
Tools for the practical work in labs; application notes
Software and Programs

General Information

An introduction - Format: PDF

HPLC Training video
The HPLC training video on this webpage describes the different parts of a HPLC instrument. A picture of how the whole system operates is built up gradually by introducing each part and defining the role that it plays. MTS, UK

Lecture Notes, Tutorials

Chiral HPLC
Basics: definitions, principles and more - Format: PDF

High Performance Liquid Chromatography
Fundamentals - Format: PDF

High Performance Liquid Chromatography (HPLC) Course
This website is intended for the academic support of HPLC/LC-MS users

High Performance Liquid Chromatography Mass Spectrometry
HPLC/MS Technique

Lecture notes - Format: PDF

HPLC Instrumentation
Lecture notes - Format: PDF

Reverse Phase HPLC Basics for LC/MS
Tutorial. IonSource, USA

Special Information

HPLC Handbook
Knauer ChromGuide is intended to assist you in all aspects of your daily HPLC tasks

Tools for the practical work in labs; application notes

Chiral Separations - Application Guide
A selection of over 1200 chiral separations is proposed and organized in a database that allows search and browse facilities

Chiral Separations by HPLC
A comprehensive guide on chiral HPLC separations - Format: PDF

Column Selector
Compare and Contrast Chromatographic Columns. ACD Labs, USA

Guide to High Performance Liquid Chromatography
... and other Liquid Chromatography Techniques

High Performance Liquid Chromatography (HPLC)

HPLC Column Void Volume
Data table

HPLC Problems
HPLC troubleshooting guide - Format: PDF

HPLC Troubleshooter
Tips and Tricks of HPLC System Troubleshooting. Agilent - Format: PDF

HPLC Troubleshooting Guide
How to identify, isolate, and correct the most common HPLC problems. Sigma-Aldrich - Format: PDF

HPLC troubleshooting Guide

Software and Programs

HPLC Simulation
Calculation form. Colby College, USA


Biomedical Chromatography
An International Journal Devoted to Research in Chromatographic Methods and Their Applications in the Biosciences. Wiley-Interscience

International Journal for Rapid Communication in Chromatography and Associated Techniques. As of 2008 no longer published by Elsevier

Journal of Chromatographic Science
... is an international scientific journal providing in-depth information on all chromatography techniques, applications, sample preparation methods, systems problem solving and more. Preston

Journal of Chromatography A
... provides a forum for the publication of original research and critical reviews on all aspects of fundamental and applied separation science. Elsevier

Journal of Chromatography B
... addresses advancements in and applications of analytical methodologies related to drugs, other biologically active compounds, metabolites, biomarkers etc. Elsevier

Journal of Liquid Chromatography ' Related Technologies
... is an internationally acclaimed forum for fast publication of critical, peer reviewed manuscripts dealing with analytical, preparative and process scale liquid chromatography and all of its related technologies. Taylor and Francis, UK

Journal of Microcolumn Separations
Last issue was published 2001. Archive. Wiley-Interscience

Journal of Separation Science
JSS is the most comprehensive source in separation science, as all areas of chromatographic and electrophoretic separation methods are covered. This includes fundamentals, instrumentation and applications, both in analytical and preparative mode. Wiley-Interscience


HPLC method to assay total saponins in Ilex paraguariensis aqueous extract
Article: J. Braz. Chem. Soc. vol.16 no.4 São Paulo July/Aug. 2005

Related Books and Scientific Literature: HPLC

Book recommendation

Michael W. Dong

Modern HPLC for Practicing Scientists

A comprehesive yet concise guide to Modern HPLC.

Written for practitioners by a practitioner, Modern HPLC for Practicing Scientists is a concise text which presents the most important High-Performance Liquid Chromatography (HPLC) fundamentals, applications, and developments. It describes basic theory and terminology for the novice, and reviews relevant concepts, best practices, and modern trends for the experienced practitioner. Moreover, the book serves well as an updated reference guide for busy laboratory analysts and researchers.

Wiley-Interscience; 2006

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Last update 30.04.2016

Keywords: High Performance Liquid Chromatography, HPLC, basics, techniques, information, application

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